Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase

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Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase

Propranolol, a widely used beta-blocker, inhibits the hydrolysis reaction of enzyme cholinesterase. Measurements of the difference in rate of hydrolysis rate between uninhibited and inhibited reactions allow the development of a kinetic method for its determination. Both systems, enzyme–substrate–chromogen and enzyme–substrate–chromogen–inhibitor, were characterized through biochemical kinetic ...

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Conductometric determination of propranolol hydrochloride in pharmaceuticals

______________________________________________________________________________ In this paper the conductometric titration of propranolol hydrochloride in pharmaceutical formulations using silver nitrate as titrant is proposed. The method was based on the formation of an insoluble salt (AgCl(s)) between the chloride of propranolol hydrochloride molecule and Ag(I) ions of the titrant AgNO3. The e...

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ژورنال

عنوان ژورنال: Hemijska industrija

سال: 2012

ISSN: 0367-598X,2217-7426

DOI: 10.2298/hemind120128032s